Different mechanisms regulate IPSC kinetics in early postnatal and juvenile
hippocampal granule cells.
Draguhn, Andreas and Uwe Heinemann.
Institut f[umlaut]ur Physiologie der Charit[acute]e, Abt. Neurophysiologie,
Tucholskystr. 2, 10117 Berlin, Germany, Tel.: ++49-30-2802-6640, Fax.: ++49-
APStracts 3:0161N, 1996.
SUMMARY AND CONCLUSIONS
1. Monosynaptic inhibitory postsynaptic currents (IPSCs) were recorded from
early postnatal and juvenile dentate granule cells in rat brain slices at room
temperature. The focally evoked currents were mediated by gamma-aminobutyric
acid receptors (GABA A -receptors). 2. IPSCs were characterized by a steep
rising phase and a slower, monoexponential decay time course. The decay time
constant was potential dependent and average values ranged from 33 ms at -60
mV to 58 ms at +40 mV holding potential. 3. IPSCs were studied in tissue from
animals between postnatal day 3 (p3) and p25. All kinetic parameters as well
as the mean current amplitude were unchanged during this ontogenetic period.
4. In juvenile granule cells from animals aged 13 to 16 days addition of the
GABA uptake blocker tiagabine (10 [mu]M) prolonged the decaying phase of the
IPSCs. The current decay remained monoexponential but the time constant t
decay increased to 250% of control values. Mean current amplitudes remained
largely unchanged. 5. In contrast, tiagabine had no effect on IPSCs in early
postnatal tissue. The decay time constant remained unchanged in cells recorded
from animals aged p4 - p6. Other uptake blockers were also ineffective during
the first postnatal week whereas [beta]-alanine, NNC-711 and L-2,3-
diaminoproprionic acid (DAPA) enhanced t decay in the older tissue (p13 -
p16). 6. Hypoosmolaric extracellular solution was applied to restrict the
extracellular space. In juvenile tissue (p13 - p16), IPSCs were not affected
by this treatment, whereas early postnatal granule cells (p4 -p6) displayed
clearly prolonged IPSC decay time constants (165% of control). 7. We conclude
that the mechanism governing the kinetics of evoked IPSCs in granule cells
changes during ontogenesis. Whereas in early postnatal tissue the transmitter
leaves the postsynaptic site by diffusion, GABA uptake becomes time-limiting
after two weeks of postnatal development.
Received 29 November 1995; accepted in final form 29 July 1996.
APS Manuscript Number J805-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 August 1996