Salt taste responses of mouse chorda tympani neurons: Evidence for existence of two different amiloride-sensitive receptor components for NaCl with different temperature dependencies. Ninomiya, Yuzo. Department of Oral Physiology, Asahi University School of Dentistry, Hozumi, Motosu, Gifu 501-02, Japan.
APStracts 3:0164N, 1996.
SUMMARY AND CONCLUSIONS
1. Inhibitory effects of amiloride on salt responses of single fibers of the chorda tympani nerve of C57BL/6 strain of mice were examined at two different temperatures (around 12 and 24C). 2. Out of 36 single fibers that responded to NaCl, 20 fibers showed strong suppression of responses to NaCl by lingual treatment with amiloride (ami-sensitive fibers), whereas the remaining 16 fibers showed no such amiloride inhibition (ami-insensitive fibers). 3. Twenty ami-sensitive fibers were further classified into two subgroups according to the temperature dependency of their NaCl responses. In 15 out of 20 fibers, amiloride inhibitable NaCl responses were larger at 24C than at 12C, whereas the reverse was true for the remaining 5 fibers. All ami-insens- itive fibers showed smaller responses to NaCl at 12C. 4. These results suggest that there exist two different amiloride- sensitive receptor components for NaCl with different temperature dependencies in mouse taste cells: one is more sensitive to NaCl at the higher temperature, and the other is more sensitive at the lower temperature.

Received 29 May 1996; accepted in final form 31 July 1996.
APS Manuscript Number J418-6.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 29 August 1996