Properties of a Slow Nonselective Cation Conductance Modulated by
Neurotensin and Other Neurotransmitters in Midbrain Dopaminergic Neurons.
Farkas, Ronald H., Pei-Yu Chien, Shigehiro Nakajima, Yasuko Nakajima.
Department of Anatomy and Cell Biology* and Department of Pharmacology(,
University of Illinois at Chicago, College of Medicine, Chicago, IL 60612.
APStracts 3:0111N, 1996.
SUMMARY AND CONCLUSIONS
1. A widespread mechanism of slow excitation throughout the nervous system
involves overlapping changes in nonselective ion conductance and K+
conductance. We used whole-cell patch clamp recording to characterize such a
nonselective conductance induced by neurotensin (NT) and other
neurotransmitters in immunocytochemically identified dopaminergic neurons
cultured from the rat ventral tegmental area (VTA). 2. The NT-induced inward
current consisted of an initial peak and later "hump". The response was
reversibly blocked by the non-peptide NT receptor antagonist SR48692,
suggesting that it resulted from activation of NT receptors. 3. The channel
was almost equally permeable to Na+ and K+, as determined from the reversal
potential shift upon switching from Na+- to K+-containing external solution.
The permeability of Cs+ was similar to that of Na+, as determined from the
zero-current equation and average reversal potential in the 75 mM Na+
solution. Cl- was not significantly permeable. 4. In Ca2+-free external
solution the NT-induced current showed a four-fold increase in amplitude, and
in high Mg2+ (20 mM) external solution the NT-induced current showed an 80%
decrease in amplitude, suggesting that external Ca2+ and Mg2+ could block the
nonselective conductance. 5. The NT response was unaffected by loading the
neurons with either the Ca2+ chelator BAPTA or with 1 mM Ca2+. The
nonselective conductance was therefore not Ca2+-activated. 6. Loading the
neurons with cyclic GMP or cyclic AMP (each with the phosphodiesterase
inhibitor IBMX) did not affect the NT response. The NT-induced nonselective
conductance was therefore not cyclic nucleotide-activated. 7. The latency of
the NT response was long ((185 msec, average 406 msec, 30°C), indicating that
NT did not induce the conductance through ligand-gated channels. Thus, NT
activated a slow nonselective cation conductance. 8. Neurokinin B, a
metabotropic glutamate agonist, and muscarine elicited responses similar to
the NT response. The NT response could be elicited after desensitizing the
responses to these other neurotransmitters, indicating receptor specificity in
the activation of the nonselective conductance.
Received 6 September 1995; accepted in final form 13 May 1996.
APS Manuscript Number J595-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 17 June 96