Sympathetic Modulation of Activity in Rat Dorsal Root Ganglion Neurons
Changes Over Time Following Peripheral Nerve Injury.
MICHAELIS, MARTIN, MARSHALL DEVOR, and WILFRID J[umlaut]aNIG.
Physiologisches Institut, Christian-Albrechts-Universit[umlaut]at, 24098
Kiel, Germany, and Department of Cell and Animal Biology, Life Sciences
Institute, Hebrew University of Jerusalem, Jerusalem 91904, Israel.
APStracts 3:0047N, 1996.
SUMMARY AND CONCLUSIONS
1 . We recorded from centrally connected axons isolated from the proximal
stump of the sciatic nerve in intact rats and in rats whose nerve had been
transected 4 days to 6 months previously. Afferent axons selected for study
had spontaneous impulse activity which originated ectopically in dorsal root
ganglia (DRGs L4 and 5). The sympathetic supply of these DRGs was excited by
repetitive electrical stimulation of ventral roots T13 and L1. We examined
quantitatively changes in afferent ongoing firing evoked by sympathetic
stimulation. Results are based on observations from 161 neurons in rats with
sciatic nerve injury, and 58 neurons from control rats with intact sciatic
nerve. Of these 219 neurons, 204 had myelinated fibers (A-neurons) and 15 had
unmyelinated fibers (C-neurons), based on measurements of conduction velocity.
2 . In rats with nerve injury the majority of the spontaneously active neurons
tested (95/161) responded to sympathetic stimulation with a change in ongoing
firing frequency: 41 neurons exhibited a significant increase in discharge
frequency which was often followed by suppression (28/41), and 54 neurons
responded with a decrease in ongoing activity (simple suppression). In control
rats, in contrast, only 1 of the 58 spontaneously active sensory neurons
tested responded to sympathetic stimulation. 3 . In A-neurons, the response
pattern changed systematically with time after sciatic nerve injury. At 4-22
days after nerve lesion, excitation was much more common than suppression. At
60-93 days, excitation and suppression occurred about equally. At 110-171 days
suppression was by far the more common response. 4 . Of the 14 C-neurons, 2
were excited by sympathetic stimulation (at 4-22 days postoperatively) and 10
were suppressed (2 at 4-22 days, 8 at >60 days). The only spontaneously active
C-neuron found in control rats was not affected by sympathetic stimulation. 5
. The magnitude of responses in the three postoperative intervals investigated
was similar. This was so for both the excitatory and the simple suppressive
responses. The average latency between onset of stimulation and excitatory
responses in afferent A-fibers (about 10s) was significantly less than the
latency to simple suppressive responses (about 20s). 6 . The mean spontaneous
firing rate of A-neurons decreased with time after nerve lesion. No change was
observed in C-neuron activity. The mean firing rate of A-neurons was
significantly higher than that of C-neurons 4-93 days after nerve lesion, but
not later. In all three postoperative periods investigated the mean rate of
spontaneous activity was the same in A-neurons that responded to sympathetic
stimulation and A-neurons that did not. 7 . The results show that nerve injury
triggers sympathetic-sensory coupling within rat DRGs. Excitatory coupling is
preferentially present in the period shortly after nerve injury, and is
subsequently replaced by suppressive coupling. This suggests that there is a
gradual change in the underlying coupling mechanism.
Received 23 January 1995; accepted in final form 21 February 1996.
APS Manuscript Number J50-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 20 March 96