Encoding of corneal input in two distinct regions of the spinal trigeminal
nucleus in the rat: cutaneous receptive field properties, responses to thermal
and chemical stimulation, modulation by diffuse noxious inhibitory controls,
and projections to the parabrachial area.
Meg, I.D., J.W. Hu, A.P. Benetti, D.A. Bereiter.
Departments of Neuroscience and Surgery, Brown University/ Rhode Island
Hospital, Providence, RI. 02903 USA, Faculty of Dentistry, University of
Toronto, Toronto, Ont. M5G 1G6 Canada
APStracts 3:0219N, 1996.
To determine if corneal input is processed similarly at rostral and caudal
levels of the spinal trigeminal nucleus, the response properties of second-
order neurons at the transition between trigeminal subnucleus interpolaris and
subnucleus caudalis (Vi/Vc), and at the transition between subnucleus caudalis
and the cervical spinal cord (Vc/C1) were compared. Extracellular single units
were recorded in 68 Sprague-Dawley rats under chloralose or
urethane/chloralose anesthesia. Neurons that responded to electrical
stimulation of the cornea at the Vi/Vc transition region (n = 61) and at
laminae I/II of the Vc/C1 transition region (n = 33) were classified
regarding: i). corneal mechanical threshold, ii). cutaneous mechanoreceptive
field, if present, iii). electrical input characteristics (A- and/or C-fiber),
iv). response to thermal stimulation, v). response to the small fiber
excitant, mustard oil (MO), applied to the cornea, vi). diffuse noxious
inhibitory controls (DNIC), and vii). projection status to the contralateral
parabrachial area (PBA). Based on cutaneous receptive field properties,
neurons were classified as low-threshold mechanoreceptive (LTM), wide dynamic
range (WDR), nociceptive specific (NS), or deep nociceptive (D). All neurons
recorded at the Vc/C1 transition region were either WDR (n = 19) or NS (n =
14). In contrast, 54% of the Vi/Vc neurons had no cutaneous receptive field.
Of those Vi/Vc neurons that had a cutaneous receptive field, 57% were LTM, 25%
WDR and 18% D. All Vc/C1 neurons responded to noxious thermal and MO
stimulation. Only 22/47 and 13/19 Vi/Vc corneal units responded to thermal or
MO stimulation, respectively. At the Vc/C1 transition region, 12/17 neurons
demonstrated DNIC, whereas at the Vi/Vc transition region, DNIC was present in
only 4/26 neurons. Twelve of 15 Vc/C1 corneal units could be antidromically
activated from the contralateral PBA (average latency = 6.29 ms, range of 1.8
to 26 ms). None of 22 Vi/Vc corneal units tested could be antidromically
activated from the PBA. These findings suggest that neurons in laminae I/II at
the Vc/C1 transition and at the Vi/Vc transition process corneal input
differently. Neurons in laminae I/II at the Vc/C1 transition process corneal
afferent input consistent with that from other orofacial regions. Corneal-
responsive neurons at the Vi/Vc transition region may be important in motor
reflexes or in recruitment of descending antinociceptive controls.
Received 14 June 1996; accepted in final form 18 September 1996.
APS Manuscript Number J478-6.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996