Dimethyl sulfoxide decreases interleukin-8 mediated neutrophil
recruitment in the airways.
Massion, P. P., A. Lind[acute]en, H. Inoue, M. Mathy, K. M. Grattan,
and J. A. Nadel.
Cardiovascular Research Institute and Department of Medicine,
University of California San Francisco, San Francisco, CA, Centre
Interdisciplinaire de Biochimie Normale et Pathologique de
l'Oxyg[grave]ene, Universit[acute]e de Li[grave]ege, Belgium
APStracts 3:0119L, 1996.
In this study, we investigated the role of dimethyl sulfoxide (DMSO)
in inhibiting Interleukin-8 (IL-8)- mediated neutrophil recruitment
induced by Pseudomonas aeruginosa?(PA) bacterial supernatant. First,
we tested whether DMSO could inhibit IL-8 production induced by PA in
human bronchial epithelial (16-HBE) cells in vitro. In these cells,
exposure to PA or H2O2 induced IL-8 production dose-dependently, an
effect that was inhibited by 1% DMSO at both the protein and RNA
level. Second, we tested whether DMSO could block the recruitment of
neutrophils induced by PA in a bypassed segment of dog trachea in
vivo. PA supernatant was placed in the tracheal segment for 6 h in
four dogs, and neutrophil recruitment and IL-8 concentrations were
measured in the superfusate. DMSO prevented the recruitment of
neutrophils and IL-8 production induced by PA time-dependently. The
results suggest that DMSO may play an anti-inflammatory role in the
airway by inhibiting IL-8 production in epithelial cells.
Received 12 October 1995; accepted in final form 24 June 1996.
APS Manuscript Number L302-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 4 August 1996