Contribution of protein kinase c to [beta] -hexosaminidase-induced
airway smooth muscle proliferation.
Lew, D. Betty, Eric R. Brown, B. Kinard Dempsey, Harold M. Wright, and
Kafait U. Malik.
Crippled Children's Foundation Research Center at LeBonheur
Children's Medical Center, Departments of Pediatrics and
Pharmacology, College of Medicine, University of Tennessee, Memphis,
Tennessee 38103
APStracts 3:0221L, 1996.
B-Hexosaminidases (Hex) promote mitogenesis via airway smooth muscle
mannose receptor (ASM-MR). The objective of this study was to
elucidate the contribution of protein kinase C (PKC) in Hex-induced
mitogenesis in ASM cells (ASMC). Exposure of ASMC to Hex caused
increase in both the calcium-dependent and the calcium-independent
PKC activities. Both down-regulation of PKC and PKC inhibitors
Staurosporine and Calphostin C diminished Hex-induced DNA synthesis
and cell number. Hex-induced DNA synthesis was enhanced by a
diacylglycerol kinase inhibitor R59022 which was blocked by
Calphostin C. These data suggest that activation of PKC in part
mediates Hex-induced mitogenesis in ASMC.
Received 25 July 1995; accepted in final form 6 November 1996.
APS Manuscript Number L230-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996