Endothelin-1 causes sequential trapping of platelets and
neutrophils in the pulmonary microcirculation in rats.
Helset, Elin, Sigurd Lindal, Randi Olsen, Reidar Myklebust, and Leif
J[stod]orgensen.
Department of Anesthesiology, Institute of Clinical Medicine, and
Department of Morphology, Institute of Medical Biology, University of
Troms[stod]o, Troms[stod]o, Norway
APStracts 3:0079L, 1996.
E.Helset, S.Lindal, R. Myklebust, R. Olsen and L.J[stod]orgensen.
Endothelin-1 causes a sequential trapping of platelets and leukocytes
in the pulmonary circulation. ----------- We previously showed that
endothelin-1 (ET-1) causes accumulation of leukocytes in the
pulmonary microvasculature, and increases vascular permeability in
isolated rat lungs provided the presence of leukocytes in the
perfusate. In the present study we examined the time-sequence for
morphological changes induced by ET-1 in rat alveolar tissue. For
this purpose we used morphometric analysis based on lung transmission
electron micrographs. Morphometry was performed by point counting,
and data expressed as relative volume density. ET-1, at 0.06, 0.6 and
6 nmol/kg was infused into the internal jugular vein, and the animals
were killed at certain points of time. The lungs were fixed by
endotracheal instillation of McDowells fixative.
Received 28 March 1995; accepted in final form 30 April 1996.
APS Manuscript Number L202-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 June 96