APStracts 3:0071L, 1996.

Protective effects of BQ123, an ETA receptor antagonist, against leukotoxin- induced injury in isolated perfused rat lungs. Ishizaki, Takeshi, Kazuo Shigemori, Shingo Ameshima, Tsuguhiko Nakai, Susumu Miyabo, Mika Hayakawa, Takayuki Ozawa, and Norbert F. Voelkel. Dept. of Internal Medicine, Fukui Medical School, Fukui, Japan, 910-11, Dept. of Biomedical Chemistry, University of Nagoya, Nagoya, Japan 466, Pulmonary Hypertension Center, Div. of Pulmonary Sciences and Critical Care, Medicine, Univ. of Colorado Health Sciences Center, Denver, CO 80262

APStracts 3:0071L, 1996.

We tested the hypothesis that BQ123, a novel ETA receptor antagonist, protects the lung against leukotoxin, 9, 10-epoxy-12-octadecenoate (Lx), which causes acute lung injury in animals. In isolated rat lungs perfused with Earle's balanced salt solution, BQ123 suppressed the Lx-induced increase in wet lung weight, wet lung weight/dry lung weight, the effluent perfusate lactic dehydrogenase (LDH) activity and effluent perfusate and lung tissue ET1 levels. BQ123 also significantly attenuated the Lx-induced increase of the pulmonary capillary filtration coefficient (Kfc). Thus, our experimental results indicate that the ETA receptor antagonist, BQ123 protects against Lx- induced experimental lung vascular injury.

Received 16 March 1995; accepted in final form 5 May 1996.
APS Manuscript Number L84-5.
Article publication pending Am. J. Physiol. (Lung,  Cell. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 May 96