Temporal changes in expression of tgf-[beta] isoforms in mouse lung
exposed to oxygen.
O'reilly, Michael A., Rhonda J. Staversky, Kathleen C. Flanders, Carl
J. Johnston, Jacob N. Finkelstein.
Department of Pediatrics (Neonatology), Children's Hospital at
Strong, University of Rochester, Rochester, NY 14642, Laboratory of
Chemoprevention, Building 41, Rm C629, National Cancer Institute,
National Institutes of Health, Bethesda, MD 20892
APStracts 3:0169L, 1996.
Oxygen-induced pulmonary injury is associated with cell death and a
significant inflammatory response. Since transforming growth factor
-[beta] is a potent modulator of the immune response, changes in
expression of the three transforming growth factor-[beta] (TGF
-[beta]1,-[beta]2,-[beta]3) isoforms was determined in lungs of adult
mice exposed to >95% oxygen. TGF-[beta]1 immunostaining within
cuboidal non-ciliated bronchiolar epithelial cells was increased
within 3 hours of oxygen exposure and continued to increase for 48
hours before decreasing to control levels by 72 hours. A similar, but
less marked change, was observed in granulated cells that were
morphologically consistent with alveolar type II cells.
Immunostaining for TGF-[beta]2 and TGF-[beta]3 revealed a similar
change in bronchiolar epithelium with little change observed in the
alveolar epithelium. Immunohistochemical changes in TGF-[beta]
expression were not observed in any other pulmonary cells. Northern
blot analysis of total lung RNA revealed that expression of the TGF-
b mRNAs were not markedly altered over the 72 hour exposure period.
Exposure to >95% oxygen resulted in cell-type specific post
-transcriptional changes in TGF-[beta] isoforms in the lung.
Received 4 June 1996; accepted in final form 29 August 1996.
APS Manuscript Number L156-6.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996