Gelatinases in epithelial lining fluid of patients with adult
respiratory distress syndrome.
Delclaux, Christophe, Marie-Pia D'ortho, Christophe Delacourt,
Fran[grave]iois Lebargy, Christian Brun-Buisson, Laurent Brochard,
Fran[grave]iois Lemaire, Chantal Lafuma, Alain Harf.
D[umlaut]apartement de Physiologie and Institut National de la
Sant[umlaut]a et de la Recherche M[umlaut]adicale, INSERM U 296,
Service de R[umlaut]aanimation M[umlaut]adicale, H[diaeresis]opital
Henri Mondor, Assistance Publique-H[diaeresis]opitaux de Paris, 94010
Cr[umlaut]ateil, France
APStracts 3:0188L, 1996.
Gelatinases A and B are matrix metalloproteinases, secreted as
inactive pro-forms and capable of degrading basement membrane
components after activation in the extracellular environment. To
determine whether the presence of gelatinases A (non-inflammatory
cells) and B (inflammatory cells) in epithelial lining fluid (ELF) is
associated with the adult respiratory distress syndrome (ARDS) we
studied 28 patients divided into four groups based on presence of
ARDS and/or multiple organ failure (MOF). Gelatinase activities were
quantified in ELF using zymography. ARDS patients had larger ELF
volumes than non-ARDS patients: 9.2 8.5 ml /100 ml of recovered
lavage fluid (n=18) versus 1.9 1.7 ml /100 ml (n=11), respectively
(p< 0.001). Presence of activated gelatinase A in ELF was a sensitive
(16/18 ARDS patients, 89%) and specific (0 /11 non-ARDS patients with
or without MOF) marker for ARDS. Activated gelatinase (A plus B)
activities /l ELF were correlated with albumin concentrations in ELF
(p< 0.01), and activated gelatinase activities in ELF were
correlated with the volume of ELF (p< 0.0005). This could
suggest an involvement of these gelatinases in alveolar-capillary
permeability increase.
Received 24 April 1996; accepted in final form 21 October 1996.
APS Manuscript Number L125-6.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 13 November 1996