Regulation of Gene Expression by Insulin.
O'BRIEN, RICHARD M. AND DARYL K. GRANNER.
Department of Molecular Physiology and Biophysics, Vanderbilt University
Medical School, Nashville, Tennessee
APStracts 2:0031P, 1996.
ABSTRACT
The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme in the
synthesis of long-chain fatty acids, is regulated allosterically and by
covalent modification (111). In addition, expression of the ACC gene is also
regulated at the transcriptional level. Two alternative promoters (designated
I and II) are utilized in a tissue-specific fashion (313). Pape and Kim (412)
have studied the induction of ACC gene expression from the type II promoter
during insulin-stimulated differentiation of 30A5 preadipocytes to adipocytes.
The degree of cell differentiation increases in parallel with ACC activity
(412). Whether this effect of insulin on ACC gene expression is direct, or
requires the insulin-stimulated synthesis of another protein during the
initiation of differentiation, is unknown. The induction of ACC mRNA by
insulin in adipose tissue explants requires the presence of glucose (170), but
whether this is also the case during insulin-stimulated 30A5 preadipocyte
differentiation is unclear.
APS Manuscript Number P13-6.
Article publication pending October 1996, Physiological Reviews.
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 13 November 1996