A comparison of the effects of food deprivation and 2, 5-anhydro-d -mannitol on metabolic parameters and ingestive behavior. Park, Collin R., Lambertus Benthem, Randy J. Seeley, Mark I. Friedman, Charles W. Wilkinson, and Stephen C. Woods. Behavioral Neuroscience Interdisciplinary Group and Depts. of Psychology and Psychiatry and Behavioral Sciences, University of Washington, Seattle, WA, 98195, Veterans Affairs Medical Centers, Seattle, WA, 98108 and Tacoma,WA, 98493, and Monell Chemical Senses Center, Philadelphia, PA, 19104
APStracts 3:0016R, 1996.
Using respiratory quotient as an index of metabolic state, we compared the effects of administration of the fructose analog 2,5-anhydro-D -mannitol (2,5-AM), at a dose of 300 mg/kg, to the effect of 10 hours of food deprivation. We measured behavioral and physiological responses of the animals receiving the two treatments, including food intake, energy expenditure, rates of carbohydrate and fatty acid utilization, and plasma levels of glucose, insulin, corticosterone, epinephrine and norepinephrine. A vehicle-treated control group was also included. Fasting produced a greater food intake than 2,5-AM administration. Although plasma glucose, insulin and norepinephrine levels were similar between the two treatments, plasma corticosterone and epinephrine levels were significantly elevated in animals receiving 2,5-AM. We conclude that although 2,5-AM can produce a metabolic state similar to fasting, as measured by an index of whole -body metabolic state (respiratory quotient), there remain factors which influence food intake which are not similar in the two conditions.

Received 26 April 1995; accepted in final form 29 December 1995.
APS Manuscript Number R253-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 22 January 96