Central infusion of leptin and glp-1-(7-36) amide differentially
stimulate c-fos-like immunoreactivity in the rat brain.
Dijk, Gertjan Van, Todd E. Thiele, Jamie C. K. Donahey, L. Arthur
Campfield, Francoise J. Smith, Paul Burn, Ilene L. Bernstein, Stephen
C. Woods, and Randy J. Seeley.
DEPARTMENTS OF PSYCHOLOGY, BEHAVIORAL NEUROSCIENCE AND MEDICINE,
UNIVERSITY OF WASHINGTON, SEATTLE, WA AND DEPARTMENT OF METABOLIC
DISEASES, HOFFMAN LAROCHE4, NUTLEY, NJ
APStracts 3:0276R, 1996.
Recently, glucagon-like peptide-1-(7-36) amide (GLP-1) and leptin have
been implicated in the regulation of food intake. In the present
study, we compared the effects of third ventricular administration
(i3vt) of leptin (3.5 mg) and GLP-1 (10.0 mg) on short-term food
intake and c-Fos-like immunoreactivity (c-FLI) in hypothalamic,
limbic and hindbrain areas in the rat. Relative to controls, infusion
of leptin or GLP-1 (3 hr before lights off) significantly reduced
food intake over the first 4 hrs in the dark phase (53% and 63%,
respectively). In different rats, infusion of leptin or GLP-1
elevated c-FLI in the paraventricular hypothalamus and central
amygdala. Furthermore, leptin selectively elevated c-FLI in the
dorsomedial hypothalamus, whereas GLP-1 selectively elevated c-FLI in
the nucleus of the solitary tract, area postrema, lateral
parabrachial nucleus and the arcuate hypothalamic nucleus. The fact
that most of the c-FLI following leptin or GLP-1 administration was
observed in separate regions within the CNS suggests different roles
for leptin and GLP-1 in the CNS regulation of food intake and body
weight.
Received 26 April 1996; accepted in final form 26 June 1996.
APS Manuscript Number R233-6.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 25 July 1996