Sensitization of the colonic response to novel stress after previous stressful experience. Stam, Ruben, Gerda Croiset, Louis M. A. Akkermans, and Victor M. Wiegant. Department of Medical Pharmacology, Rudolf Magnus Institute for Neurosciences, Utrecht University, 3508 TA Utrecht, The Netherlands
APStracts 3:0231R, 1996.
Stressful life events may be important causative or precipitating factors for functional gastrointestinal disorders such as the irritable bowel syndrome in humans. In the rat, a single session of footshocks is known to sensitize the behavioral and hormonal responses to subsequent stress, but intestinal responses have not been investigated. Rats were fitted with bipolar electrodes on proximal colon and exposed to a single session of footshocks (10 _ 6 s in 15 min, preshocked) or no shocks (control). Weight gain after footshocks was identical to that in controls. Two weeks after footshocks, basal colonic spike burst frequency did not differ from controls or from that recorded before shock treatment. Unlike controls, however, preshocked rats showed a significant increase in colonic spike burst frequency to a novel stressful challenge in the home cage, an electrified prod. Since the behavioral responses to this challenge did not differ, colonic hyperresponsiveness in preshocked rats may represent a form of stress-induced autonomic sensitization. The model should be a useful tool to study mechanisms and pharmacotherapeutic approaches of the gastrointestinal consequences of traumatic stress.

Received 18 December 1995; accepted in final form 13 May 1996.
APS Manuscript Number R799-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 June 96