Regional hepatic ischemia/reperfusion induces a distinct pattern of
heat shock and acute phase gene expression between ischemic and
nonischemic regions.
Gingalewski, Cynthia, Nicholas G. Theodorakis, Jay Yang, Stephen C.
Beck, and Antonio De Maio.
Johns Hopkins University School of Medicine, Baltimore, MD
21187
APStracts 3:0100R, 1996.
The hepatic response to injury is orchestrated by the expression of
different gene groups (i.e. heat shock, acute phase). In the present
study, the expression of heat shock and acute phase genes was
analyzed in the context of a localized injury, regional hepatic
ischemia/reperfusion. Left and median liver lobes were subjected to
one hour of ischemia, while blood flow was maintained to the
remainder of the organ. After the period of ischemia the organ was
reperfused, and samples of the ischemic and nonischemic liver were
obtained at different times points during reperfusion. Expression of
the heat shock gene, hsp-72, was detected only in the ischemic liver,
whereas expression of the acute phase gene, [beta]-fibrinogen, and
the interleukin-6 inducible gene, metallothionein, was maximally
induced in the nonischemic liver and attenuated in the ischemic
liver. To determine how the heat shock and acute phase responses were
reprioritized during stress, expression of [beta]-fibronogen and
hsp72 was induced simulatneously in the same animal by administration
of endotoxin and total body hyperthermia respectively. Administration
of endotoxin did not impede the expression of hsp72; however, heat
shock attenuated, but did not eliminate, the endotoxin-induced
expression of [beta]-fibronogen . These observations suggest that the
heat shock and acute phase responses are not mutually exclusive.
Received 18 November 1995; accepted in final form 5 March 1996.
APS Manuscript Number R715-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 20 March 96