The effect of electrical stimulation patterns on glucose transport
in rat muscles.
J[grave]uhannsson, E., J. Jensen, K. Gundersen, H. A. Dahl, and A.
Bonen.
The Norwegian University of Physical Education and Sport, P.O.Box
40 Kringsj[circumflex]i 0807 Oslo, Norway, Department of Anatomy,
Institute of Basic Medical Sciences, University of Oslo, 0317 Oslo,
Norway, Department of Neurophysiology, Institute of Basic Medical
Sciences University of Oslo, 0317 Oslo Norway, Department of
Kinesiology, University of Waterloo, Waterloo, Ontario, N2L 3G1
Canada
APStracts 3:0110R, 1996.
Transport of [3H]-2-deoxy-D-glucose (2-DG) was investigated during
supramaximal stimulations of different muscles. In addition, we
varied the the net stimulation time (NST). In different treatments
NST occupied either 5, 7.5, 10, 15, 20, 30, or 50% of a 20 min
stimulation period. After a bolus injection of [3H]-2-DG, the
greatest transport occurred in the extensor digitorum longus (EDL).
In the red gastrocnemius (RG; type IIa fibers) and the white
gastrocnemius (WG; type IIb fibers) the 2-DG transport rate was
highest at 10% NST (8-12 fold increase) and decreased thereafter. In
soleus (type I fibers) the 2-DG transport increased from 5% to 50%
NST. Below 30% NST, 2-DG transport was greater in RG and WG muscles
than in soleus (P<0.05). GLUT-4 and 2-DG transport were not
correlated during the contractions. Therefore, the %NST affects 2-DG
transport differentially in muscles of varying fiber types and the
transport rate is not related to the GLUT-4 content of the muscles.
Received 5 June 1995; accepted in final form 19 February 1996.
APS Manuscript Number R343-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 27 March 96