Role of the renin-angiotensin system in mediating the effects of posture on renal function. Reinhart, Glenn A., and Thomas E. Lohmeier. University of Mississippi Medical Center, Department of Physiology and Biophysics, 2500 North State Street, Jackson, MS 39216-4505
APStracts 3:0074R, 1996.
This study was designed to quantitate the influence of the neurohumoral activation associated with orthostatic stress on renal hemodynamics and sodium excretion, and further, to determine the importance of the renin-angiotensin system in mediating these changes in renal function. 7 conscious dogs were studied while lying in the recumbent position and, subsequently, after standing in a supporting sling. Experiments were conducted under control conditions and after plasma angiotensin II (ANG II) concentration was fixed at control levels by chronic infusion of captopril (14 [mu]g/kg/min) and ANG II (0.5+/-0.02 ng/kg/min). During control experiments, 45 minutes of standing increased plasma renin activity two-fold, while mean arterial pressure, heart rate, and plasma norepinephrine concentration remained unchanged. During standing, GFR and renal plasma flow (RPF) fell to 88+/-2 and 77+/-3% of recumbent values, respectively, while filtration fraction (FF) increased 16+/-1%. Additionally, urinary (UNaV) and fractional sodium excretion (FENa ) decreased to 27+/-6% and 30+/-7% of recumbent values, respectively. When plasma ANG II concentration was fixed at control levels during standing there were no significant changes in GFR, while increments in FF and reductions in RPF, UNaV, and FENa, were attenuated by 63, 40, 30, and 33%, respectively. These data suggest that in conscious dogs, standing in a supporting sling causes reflex activation of the sympathetic nervous and renin-angiotensin systems, eliciting reductions in GFR, RPF, and urinary sodium excretion. Furthermore, ANG II contributes significantly to the effects of passive standing on renal hemodynamics and urinary sodium excretion.

Received 10 September 1995; accepted in final form 23 February
1996.
APS Manuscript Number R561-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 13 March 96