Anorexia following chronic infusion of islet amyloid polypeptide in
rats.
Arnelo, Urban, Johan Permert, Thomas E. Adrian, J[diaeresis]orgen
Larsson, Per Westermark, Roger D. Reidelberger.
Department of Biomedical Sciences, Creighton University School of
Medicine, Omaha, NE 68178; Research Service (151), Department of
Veteran Affairs Medical Center, Omaha, NE 68105, Arvid Wretlind
Laboratory for Metabolic Research, Department of Surgery, Karolinska
Institutet at Huddinge University Hospital, S-141 86 Huddinge,
Sweden; and Department of Pathology4, University Hospital,
Link[diaeresis]oping University, S-581 85 Link[diaeresis]oping,
Sweden
APStracts 3:0185R, 1996.
Islet amyloid polypeptide (IAPP) is a hormonal peptide that at high
doses has been shown to reduce food intake. In the present study, the
dose-response effects of subcutaneous infusion of IAPP (0, 2, 7 and
25 pmol/kg/min) for 8 days on food intake and meal patterns in rats
were investigated. At the end of the experiment, plasma was obtained
and levels of IAPP were measured by radioimmunoassay. IAPP dose
-dependently and transiently inhibited food intake. The minimal
effective dose (2 pmol/kg/min) caused a small but significant (up to
14%, p<0.01) inhibition of food intake which lasted 5 days. The
highest dose administered (25 pmol/kg/min) had the greatest effect
(up to 44%, p<0.001), which lasted throughout the 8 day period.
Reductions in feeding during light and dark phases occurred through a
decrease in number of meals consumed rather than meal size or meal
duration. IAPP also decreased body weight gain and water intake dose
-dependently. IAPP infusion of 2, 7, and 25 pmol/kg/min increased
plasma IAPP concentrations from a basal level of 10.3+/-0.7 pM to
35.1+/-5.4, 78.1+/-11.2, and 236.6+/-23.6 pM, respectively, values
that are likely to be close to physiological and within the
pathophysiological ranges. Thus IAPP may play an important
physiologic or pathophysiologic role in control of food intake.
Received 22 November 1995; accepted in final form 3 May 1996.
APS Manuscript Number R733-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 28 May 96