Sympatho-adrenomedullary activation cannot fully account for increased plasma renin levels during water deprivation. Blair, M. L., P. D. Woolf, and S. Y. Felten. Departments of Physiology, Medicine, and Neurobiology and Anatomy, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642
APStracts 3:0361R, 1996.
This study was designed to determine if the increase in plasma renin activity (PRA) which occurs during water deprivation is mediated by the renal sympathetic nerves or adrenomedullary catecholamine release. Male Sprague-Dawley rats were studied while conscious and unrestrained. In intact or sham-operated rats, 48 hrs of water deprivation resulted in a >3-fold increase in PRA and plasma renin concentration (PRC), but no significant change in plasma norepinephrine or epinephrine concentration. Renal denervation decreased basal PRA, reduced the magnitude of the dehydration-induced PRA increase by 33%, and abolished the renin-suppressing effect of l -propranolol infusion in water-deprived rats. Adrenal demedullation also reduced basal and water-deprived PRA and PRC. However, even the combination of renal denervation and adrenal demedullation did not prevent a significant renin response to dehydration (control PRA of 1.8+ 0.6 ng/ml/hr to dehydration PRA of 6.8+1.3 ng/ml/hr, p<.05). Therefore, some mechanism in addition to sympathetic activation plays a major role in mediating increased PRA during water deprivation.

Received 20 February 1996; accepted in final form 23 September
1996.
APS Manuscript Number R100-6.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996