Enalapril and captopril enhance antioxidant defenses in mouse
tissues.
Cavanagh, Elena M. V. De, C[acute]esar G. Fraga, Le[acute]on Ferder,
and Felipe Inserra.
Physical Chemistry, School of Pharmacy and Biochemistry, University
of Buenos Aires, 1113-Buenos Aires, and 2Institute of Nephrology,
Jewish Hospital, and Argentine Hebrew University Bar-Ilan, CIMAE,
Buenos Aires-1416, Argentina
APStracts 3:0337R, 1996.
This study was conducted to investigate a possible systemic effect of
ACE inhibitors (ACEi) on tissue antioxidant defenses. CF1 mice (4
-month old females) were administered either water (control) or water
containing enalapril (20 mg/l) or captopril (50 mg/l) during 11
weeks. Neither enalapril nor captopril treatment had an effect on
body mass, brain, kidney or heart weight relative to controls. CuZn
-superoxide dismutase (CuZn-SOD) activity was increased by enalapril
treatment in kidney medulla (27%), heart (24%), and erythrocytes
(19%), and by captopril treatment in kidney medulla (43%) and heart
(54%) relative to controls. Mn-superoxide dismutase (Mn-SOD) and
catalase activities were unaffected by either treatment. Enalapril,
but not captopril treatment, increased Se-glutathione peroxidase (Se
-GPx) activity in renal medulla (19%). Non-enzymatic antioxidant
defenses, evaluated by tert-butyl hydroperoxide-initiated
chemiluminescence (HICL), were enhanced in kidney cortex (48%) by
enalapril, and in brain by enalapril (44%) or captopril (36%)
treatment relative to controls. As evaluated in vitro by HICL and
thiobarbituric acid-reactive substances (TBARS) formation, captopril
had a free radical scavenger activity, whereas neither enalapril nor
lisinopril were effective. These results suggest that ACEi may
protect tissues from oxidative damage by increasing enzymatic and
non-enzymatic antioxidant defenses.
Received 15 April 1996; accepted in final form 27 August 1996.
APS Manuscript Number R212-6.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 19 September 1996