Human leptin has natriuretic activity in the rat.
Jackson, Edwin K., and Ping Li.
Center for Clinical Pharmacology, Departments of Pharmacology and
Medicine, University of Pittsburgh Medical Center, Pittsburgh,
Pennsylvania 15213-2582
APStracts 3:0219F, 1996.
The purpose of this study was to determine whether leptin influences
renal function. Increasing doses (0.3, 1, 3, 10 and 30 [mu]g/min; 30
minutes per dose) of human leptin (h-leptin) infused into the renal
artery of anesthetized rats caused a two-fold increase
(p<0.01) in urine volume (UV), sodium excretion rate (UNaV)
and the ratio of U NaV to potassium excretion rate (U KV) from the
ipsilateral kidney, but had no effect on arterial blood pressure,
renal blood flow, glomerular filtration rate or UKV. Mouse leptin was
inactive in the rat. In a second study, a single dose of h-leptin (30
[mu]g/min) infused into the renal artery caused a significant two
-fold increase in UV and UNaV from the ipsilateral, but not
contralateral, kidney and revealed a time-lag (approximately 1.5
hours) in the measurable response. In a third study, single doses of
h-leptin were infused into the renal artery of five groups of rats
(0, 0.3, 1, 3, and 10 [mu]g/min) for 140 minutes. The ratio of U NaV
to U KV from the ipsilateral kidney was significantly increased by
all doses of h-leptin. Conclusion: h-Leptin may function as a
potassium sparing diuretic/natriuretic factor.
Received 21 February 1996; accepted in final form 13 November
1996.
APS Manuscript Number F54-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 31 December 1996