Differential localization of prostaglandin e receptor subtypes in
human kidney.
Breyer[tilde]n, Matthew D., Linda Davis, Harry R. Jacobson, Richard M.
Breyer.
Division of Nephrology, and [alpha]Department of Pharmacology,
[tilde]nDepartment of Molecular Physiology and Biophysics, Veterans
Administration Medical Center, and Vanderbilt University School of
Medicine, Nashville, Tennessee USA 37232-2372
APStracts 3:0028F, 1996.
Four PGE2 receptors designated EP1, EP2, EP3, and EP4, have been
pharmacologically identified, cloned and sequenced. The present
studies determined the intra-renal distribution of these EP receptor
subtypes in human kidney using in situ hybridization using riboprobes
for the human EP receptors. Messenger RNA for the
phosphatidylinositol hydrolysis coupled EP1 receptor was highly
expressed in cortical, outer medullary and inner medullar collecting
duct. RNA for the Gi coupled EP3 receptor was primarily expressed in
the cortical and outer medullary collecting duct, as well as in the
medullary thick ascending limb, however it was absent from the inner
medullary collecting duct. Expression of mRNA for EP1 and EP3 in
connecting segment could not be excluded. There was no expression of
the Gs-coupled EP2 receptor mRNA detected in human kidney by in situ
hybridization, however mRNA for the Gs-coupled EP4 receptor was
highly expressed in the glomerulus. These studies demonstrate
distinct regions of intra-renal expression for the different EP
-receptors and suggest that each receptor subtype may modulate
different aspects of renal function in man.
Received 21 September 1995; accepted in final form 31 January
1996.
APS Manuscript Number F320-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 14 February 96