Tubulogenesis from isolated single cells of adult mammalian kidney:
clonal analysis with a recombinant retrovirus.
Humes, H. David, John C. Krauss, Deborah A. Cieslinski, Angela J.
Funke.
Departments of Internal Medicine, VA Medical Center, and University
of Michigan
APStracts 3:0008F, 1996.
The adult mammalian kidney tubule epithelium exists in a relatively
dormant, slowly replicative state but has a large potential for
regenerative morphogenesis following severe ischemic or toxic injury.
Under selective serum free growth conditions which included epidermal
growth factor and retinoic acid, a subpopulation of renal proximal
tubule cells isolated from adult rabbit kidney were grown in cell
culture. These cells possessed two important characteristics: (i) an
ability to differentiate morphogenically into tubule structures when
grown in three-dimensional collagen gels, and (ii) a high capacity
for self renewal, since cell lineage analysis with a recombinant
retrovirus demonstrated that in vitro tubulogenesis arose from clonal
expansion of a single cell. Thus, individual cells in the adult
kidney have retained the ability for kidney tubulogenesis in vitro.
Received 13 February 1995; accepted in final form 21 December
1995.
APS Manuscript Number F51-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 22 January 96