Uninephrectomy reducecs apoptotic cell death and enhances renal
tubular cell regeneration in ischemic acute renal failure in
rats.
Nakajima, Toshiaki, Takehiko Miyaji, Akihiko Kato, Naoki Ikegaya,
Tatsuo Yamamoto, and Akira Hishida.
Department of medicine, Hamamatsu University School of
Medicine[umlaut]uAHanda-cho, Hamamatsu, 431-31, Japan
APStracts 3:0121F, 1996.
Contralateral uninephrectomy attenuates unilateral ischemic renal
injury functionally and morphologically. In this study we
investigated the effects of uninephrectomy on apoptotic renal cell
death and tubular regeneration in ischemic acute renal failure in
rats. Unilateral ischemic injury was provoked by a 60-minute left
renal artery occlusion in right nephrectomized(Nx) and sham
-nephrectomized(sham-Nx) rats. Uninephrectomy attenuated tubular
damage 48 hr following the renal ischemia. Apoptotic cells were found
in renal tissue as early as 3 hr after reperfusion and increased in
number by 12 hr. The "ladder" pattern of DNA fragments on
agarose gel electrophoresis was also apparent in ischemic kidney.
Uninephrectomy reduced apoptotic cells and DNA fragmentation. The
expression of proliferating cell nuclear antigen(PCNA) could be seen
24 hr after reperfusion and progressively increased thereafter. PCNA
expression in ischemic kidney was greater in Nx than sham-Nx rats at
24 hr after renal reperfusion. These data suggest that uninephrectomy
reduces apoptotic cells and DNA fragmentation and enhances PCNA
expression. The reduced apoptotic cell death and enhanced cell
regeneration may be importantly involved in the uninephrectomy
-induced attenuation of ischemic acute renal failure in rats.
Received 6 February 1996; accepted in final form 28 June 1996.
APS Manuscript Number F39-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 25 July 1996