Prostaglandins stimulate renin secretion and renin mrna in mouse
renal juxtaglomerular cells.
Jensen, Boye L., Charlotte Schmid, and Armin Kurtz.
Institute of Physiology, University of Regensburg, Germany
APStracts 3:0100F, 1996.
This study examined 1) effects of prostaglandins (PG) on renin
secretion and renin gene expression from isolated juxtaglomerular,
granular cells 2) expression of cyclooxygenases in juxtaglomerular
structures. Incubation of granular cell cultures with prostaglandins
E2, I2, F2[alpha],and Thromboxane B2 identified PGI2 and PGE2 as
stimulators of renin secretion; the effects were dose- and time
dependent. PGE2 also increased renin mRNA accumulation time- and
dose-dependent. PGE2 and PGI2 activated adenylate cyclase
concentration-dependent in granular-cells. PGE2-stimulations of renin
secretion and renin mRNA were non-additive to those of forskolin and
were inhibited by endothelin. The findings are compatible with
cellular actions through cAMP. On total RNA harvested from whole
kidneys, from microdissected glomeruli with attached afferent
arterioles and from mesangial cells in primary culture, RT-PCR
revealed significant expression of Cyclooxygenase I and II. By direct
interaction with PG receptors on renal juxtaglomerular cells PGE2 and
PGI2 can act as potent and rapid stimulators of renin secretion and
renin mRNA probably through cAMP-dependent pathways.
Received 13 September 1995; accepted in final form 15 May 1996.
APS Manuscript Number F313-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 June 96