Novel mouse embryonic renal marker gene products differentially
expressed during kidney development.
Kretzler, Matthias, Guang Fan, Demian Rose, Lois J. Arend, Josephine
P. Briggs, and Lawrence B. Holzman.
Division of Nephrology, Department of Internal Medicine, The
University of Michigan Medical School, Ann Arbor, Michigan 48109
-0676
APStracts 3:0102F, 1996.
Investigators approaching the problem of renal organogenesis have been
hampered by a paucity of suitable molecular markers that specify
distinct developmental phenotypes. To identify such markers,
differential display-PCR (DD-PCR) was used to survey the temporal
pattern of gene expression in mouse kidney at 11.5, 13.5, 15.5, and
17.5 days after conception and in the adult kidney. Twenty-two
differentially expressed amplification products were identified,
isolated, and sequenced. Seventeen clones showed no significant
similarity with previously reported nucleotide sequences; two were
similar to two housekeeping gene products, and three were similar to
human or rat expressed sequence tags. To confirm the differential
expression patterns observed by DD-PCR, semi-quantitative reverse
transcription-PCR was performed using sequence-specific
oligonucleotide primers. Nineteen of 22 clones were differentially
expressed during kidney development (MERM 1-19). The value of MERMs
as developmental markers was further assessed in mouse metanephric
organ culture where the pattern of MERM transcript expression
mimicked that observed in vivo. Therefore, the DD-PCR method
permitted development of a panel of marker sequences that can be used
to characterize renal developmental processes and that may allow the
identification of novel, functionally relevant gene products.
Received 21 February 1996; accepted in final form 16 May 1996.
APS Manuscript Number F60-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 June 96