Insulin-like growth factor-i action on glucose transport in acute renal failure. Mak, Robert H. K., and Otto Mehls. DEPARTMENT OF PEDIATRICS, STANFORD UNIVERSITY SCHOOL OF MEDICINE, STANFORD, CA., U.S.A.;, DIVISION OF PEDIATRIC NEPHROLOGY, UNIVERSITY CHILDREN'S HOSPITAL, HEIDELBERG, FEDERAL REPUBLIC OF GERMANY
APStracts 3:0079F, 1996.
Insulin resistance is common in acute renal failure (ARF). Insulin -like growth factor (IGF)-I has insulin-like actions. The present study is undertaken to determine whether effects of IGF-I on glucose transport are maintained in rats with ARF and to compare these actions of IGF-I with those of insulin. ARF was induced in male Sprague Dawley rats by bilateral ureteric ligation and vascular catheters were inserted at the same time in all animals. Sham operated controls were pair-fed and metabolic studies were performed 48 hours later. ARF rats had higher serum creatinine (1.3 + 0.2 mg/dl Vs 0.3 + 0.1); lower arterial pH (7.15 + 0.03 Vs 7.35 + 0.02) and higher fasting serum glucose (150 + 13 mg/dl Vs 123 + 12) compared with control rats (p &LT 0.001 in all cases). The hypoglycemic effects of insulin and IGF-I were compared using euglycemic clamps. In control rats, rates of total body glucose uptake were not different during euglycemic insulin clamps at 2 mU.kg-1.min-1 and euglycemic IGF-I clamps at 5 [mu]g.kg-1.min-1. In rats with ARF, total body glucose uptake during euglycemic insulin clamps at 2 mU.kg-1.min-1 was significantly reduced (6.5 + 0.5 mg/kg/min) compared with controls (10.5 + 1.5 mg.kg-1.min-1; p&LT0.01). In contrast, total body glucose uptake during euglycemic IGF-I clamps at 5 [mu]g.kg-1.min-1 in ARF rats (10.1 + 1.2 mg.kg-1.min-1) was not different from the corresponding value in control animals (10. 3 + 1.3 mg.kg-1.min-1). Hepatic glucose production was suppressed by insulin equally but not suppressed by IGF-I in both groups. In a second experiment, ARF was induced by bilateral renal ischemia. The following groups of animals were studied: ARF + NaCl, ARF + NaHCO3 and Control + NaCl. Insulin and IGF-I clamps were performed as above. Correction of acidosis partially corrected insulin resistance but did not affect IGF-I sensitivity. Thus the capacity of IGF-I infusion to stimulate glucose uptake is maintained in ARF rats, which are insulin resistant. 

Received 10 November 1996; accepted in final form 29 March 1996.
APS Manuscript Number F381-5.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 1 May 96