Expression of aqp family in rat kidneys during the development and
maturation.
Yamamoto, Tadashi, Sei Sasaki, Kiyohide Fushimi, Kenichi Ishibashi,
Eishin Yaoita, Katsutoshi Kawasaki, Shidehiko Fujinaka, Fumiaki
Marumo, Itaru Kihara.
Department of Pathology, Institute of Nephrology, Niigata
University School of Medicine, Niigata, and Second Department of
Internal Medicine, Tokyo Medical and Dental University, Tokyo,
Japan
APStracts 3:0188F, 1996.
The mRNA expression and localization of AQP family in rat kidney were
examined by ribonuclease protection assay and immunohistochemistry.
AQP1, AQP2, AQP3 and AQP4 mRNA were hardly detectable in 16-day
fetuses. AQP1 mRNA was explosively expressed at 1 week, keeping the
level throughout life. AQP2 mRNA expression was apparently noticed in
18-day fetuses and was enhanced gradually with age to reach a plateau
at 4 weeks. AQP3 and AQP4 mRNA expression was significantly found at
birth but was not changed remarkably thereafter. AQP2 protein
appeared first at the apical side of collecting duct cells in 18-day
fetuses. The staining intensity at the site increased with age and
basolateral staining was added in adult rats. AQP3 was distinctly
demonstrated at the basolateral side of collecting duct cells after
birth and the staining intensity was almost stable throughout life.
The progressive induction of AQP2 expression in the first 4 weeks
after birth is presumed to contribute to the maturation of urinary
concentrating capacity during the kidney development.
Received 4 March 1996; accepted in final form 8 October 1996.
APS Manuscript Number F76-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 5 November 1996