Spatial and temporal expression of cell surface molecules during
nephrogenesis.
Goldberg, Michael, Jonathan Barasch, Ashkan Shifteh, Vivette D'agati,
Juan Oliver, Chuan Hu, and Qais Al-Awqati.
Departments of Pediatrics, Medicine and Pathology, College of
Physicians and Surgeons of Columbia University, New York, New York
10032
APStracts 3:0172F, 1996.
Cell to cell interaction is fundamental to the development of the
kidney. Ureteric bud cells, through cell contact or short distance
interactions, induce the metanephric mesenchyme to convert to
epithelia and begin the process of tubulogenesis. To identify new
molecules that are involved in these processes we generated a panel
of monoclonal antibodies to the surface of induced mesenchymal cells
taken from a day 15 rat embryonic kidney rudiment. Monoclonal
antibodies were chosen for further study based either on a
distinctive pattern of expression of their antigens or their
functional effect on tubulogenesis. We identified a set of monoclonal
antibodies that preferentially stained the glomerular crevice, the
first site for formation of the glomerular anlage. Another monoclonal
antibody inhibited tubulogenesis by producing widespread apoptosis in
induced mesenchymal cells. This approach promises to identify new
molecules that are central to kidney development.
Received 22 February 1996; accepted in final form 27 August 1996.
APS Manuscript Number F63-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 7 October 1996