Endothelial cell targeting during renal development: use of
monoclonal antibodies.
Oliver, Juan A., Michael Goldberg, and Qais Al-Awqati.
Columbia University, College of Physicians & Surgeons, Dept. of
Medicine, 630 W. 168th Street, New York, NY 10032
APStracts 3:0173F, 1996.
Development of the different renal capillary beds requires that the
transformation of the metanephrogenic mesenchyme and ureteric bud
into the different nephron segments be temporally and spatially
coordinated with the migration and growth of the endothelial cells
present in the renal anlage. This suggests that ureteric bud and/or
metanephrogenic mesenchymal cells provide molecules which guide
endothelial cells to their appropriate locations. We found that
monoclonal antibody 5B6E4, obtained with mechanically dispersed cells
of E15 rat renal anlage, identifies an antigen that is temporally and
spatially associated with endothelial cell location and migration
during renal development. Further, 5B6E4 disrupts the close
association between ureteric bud ampullae and endothelial cells in
E14 renal anlages grown in vitro: while 43 % of the ureteric bud
ampullae were in contact with endothelial cells in control
conditions, the presence of 20 [mu]g/ml 5B6E4 reduced this number to
22 % (p < 0.02). These results suggest that the antigen
recognized by 5B6E4 is involved in endothelial cell targeting during
renal development.
Received 22 February 1996; accepted in final form 27 August 1996.
APS Manuscript Number F64-6.
Article publication pending Am. J. Physiol. (Renal Fluid Electrolyte
Physiology).
ISSN 1080-4757 Copyright 1996 The American Physiological Society.
Published in APStracts on 7 October 1996