APStracts 4:0185E, 1997.

Expression of a single gene produces both forms of skeletal muscle cyclic nucleotide-gated channels. Santy, Lorraine C., and Guido Guidotti. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138

APStracts 4:0185E, 1997.

Cyclic nucleotide-gated cation channels in skeletal muscle are responsible for insulin activated sodium entry into this tissue (J. E. M. McGeoch and G. Guidotti. J. Biol. Chem. 267: 832-841, 1992). These channels have previously been isolated from rabbit skeletal muscle by 8-Br-cGMP affinity chromatography, which separates them into two populations differing in nucleotide affinity (L. C. Santy and G. Guidotti. Am J Physiol 271: E1051-E1060, 1996.). In this study, a PCR approach was used to identify skeletal muscle cyclic nucleotide gated channel cDNAs. Rabbit skeletal muscle expresses the same cyclic nucleotide gated channel as rabbit aorta (M. Biel et. al, FEBS Lett. 329: 134-8, 1993). The entire cDNA for this gene was cloned from rabbit skeletal muscle, and an antiserum to this protein produced. Expression of this cDNA produces a 63 kDa protein with cyclic nucleotide-gated channel activity. A similarly sized immunoreactive protein is present in sarcolemma. Purification of the expressed channels reveals that this single gene produces both native skeletal muscle channel populations.

Received 10 June 1997; accepted in final form 7 August 1997.
APS Manuscript Number E269-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 27 August 1997