Contraction duration affects metabolic energy cost and fatigue in skeletal muscle. Hogan, Michael C., Erica Ingham, and S. Sadi Kurdak. Division of Physiology, Department of Medicine, University of California, San Diego, La Jolla, CA 92093-0623
APStracts 4:0257E, 1997.
It has been suggested that during a skeletal muscle contraction the metabolic energy cost at the onset may be greater than the energy cost related to holding steady state force. The purpose of the present study was to investigate the effect of contraction duration on the metabolic energy cost and fatigue process in fully perfused contracting muscle in situ. Canine gastrocnemius muscle (n=6) was isolated and 2 contractile periods (3 min of isometric, tetanic contractions with 45 min rest between) were conducted by each muscle in a balanced order design. The 2 contractile periods had stimulation patterns that resulted in a 1:3 contraction-to-rest ratio, with the difference in the 2 contractile periods being in the duration of each contraction: short duration=0.25 sec stimulation/0.75 sec rest vs. long duration=1 sec stimulation/3 sec rest. These stimulation patterns resulted in the same total time of stimulation, number of stimulation pulses, and total time in contraction for each 3-min period. Muscle O2 uptake, the fall in developed force (fatigue), the O2 cost of developed force, and the estimated total energy cost (ATP utilization) of developed force were significantly greater (P<0.05) with contractions of short duration. Lactate efflux from the working muscle and muscle [lactate] were significantly greater with contractions of short duration, such that the calculated energy derived from glycolysis was three times greater in this condition. These results demonstrate that contraction duration can significantly affect both the aerobic and anaerobic metabolic energy cost and fatigue in contracting muscle. In addition, it is likely that the greater rate of fatigue with more rapid contractions was a result of elevated glycolytic production of lactic acid.

Received 8 September 1997; accepted in final form 11 November
1997.
APS Manuscript Number E426-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 12 December 1997