Population-based modeling to demonstrate extrapancreatic effects of tolbutamide. Rostami-Hodjegan, A, Sr Peacey, E George, Sr Heller, and Gt Tucker. University of Sheffield Department of Medicine and Pharmacology, The Royal Hallamshire Hospital, Sheffield, S102JF. University Department of Medicine, Clinical Sciences Centre and Diabetic Centre, Northern General Hospital, Sheffield, S5 7AU, UK
APStracts 4:0271E, 1997.
Tolbutamide is used increasingly as an investigative tool in in vivo studies of the physiology of glucose tolerance. Its hypoglycemic effect in non-diabetic subjects is widely variable reflecting possible variability in its pharmacokinetics, insulinergic response, an extrapancreatic effect of the drug or the hypoglycemic effect of insulin itself. Using population-based modelling, we have investigated the kinetics and dynamics of tolbutamide and assessed covariates in two groups of healthy subjects. The results indicate a high variability in insulinergic effect, measured by the AUC of insulin (0-60 min) in response to tolbutamide injection (29-96% CV). However, it appears that impaired insulin sensitivity is compensated by higher insulin secretion in response to tolbutamide. Thus the hypoglycemic effect of high insulin secretion is minimal in insulin resistant subjects. Application of the model indicated that tolbutamide has appreciable extrapancreatic effects mediated by prolongation of the residence time of insulin in a remote effect and by enhancement of glucose effectiveness (GE). An effect in increasing the insulin sensitivity index (ISI) is also possible but could not be confirmed statistically for all of the groups of subjects studied. These observations may explain inconsistencies between the results of tolbutamide-injection and insulin-injection in the frequently sampled i.v. glucose tolerance test (FSIGT), and call for further study of insulin-modified FSIGT vs tolbutamide modified FSIGT in the assessment of ISI and GEI. (Accepted for the Modeling in Physiology Section)

Received 26 November 1996; accepted in final form 25 November
1997.
APS Manuscript Number E589-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 12 December 1997