Urate uptake and lowered atp levels in human muscle after high
intensity intermittent exercise.
Hellsten, Y., B. Sj[diaeresis]odin, E. A. Richter, and J. Bangsbo.
Copenhagen Muscle Research Centre, August Krogh Institute,
Copenhagen, Denmark, National Defense Research Establishment, and
Department of Physiology and Pharmacology, Karolinska Institute,
Stockholm, Sweden.
APStracts 4:0274E, 1997.
The exchange of purines in exercised and rested muscle and their
relation to muscle ATP levels following intense intermittent exercise
was investigated. Seven subjects performed one-legged knee extensor
exercise on two occasions; without (Control; C) and with (High
purines; HP) additional arm-exercise. There was a greater net release
of hypoxanthine by the exercised muscle during the recovery period in
HP compared to C (185+/-44 [Mean+/-SE] vs. 101+/-30 [mu]mol/kg
muscle; P<0.05). During recovery the arterial urate concentration
was higher in HP compared to C (peak: 585+/-48 vs. 355+/-20
[mu]mol/l; P<0.05). The exercised but not the rested muscle
extracted a marked amount of urate (330 [mu]mol/kg muscle) from
plasma in the HP trial. Muscle ATP levels after 90 min of recovery in
HP were lower than at rest (24.3+/-0.6 vs. 20.1+/-1.1 mmol/kg d.w.).
The present data suggests that a single session of long-term high
intensity intermittent exercise causes a significant release of
purines from the muscle into blood which contributes to a sustained
lowered level of the muscle ATP concentration. Furthermore, intensely
exercised muscle extracts urate when plasma urate is elevated, an
event which may be of importance for the replenishment of oxidized
muscle urate stores.
Received 7 October 1997; accepted in final form 9 December 1997.
APS Manuscript Number E478-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 7 January 1998