17 -estradiol modulation of glucose transporter 1 (glut 1)
expression in blood-brain barrier.
Shi, Jiong, and James W. Simpkins.
Center for the Neurobiology of Aging and Department of
Pharmacodynamics, College of Pharmacy, University of Florida,
Gainesville, FL 32610
APStracts 4:0035E, 1997.
The present study was designed to evaluate 17 -estradiol (E2)
modulation of glucose transporter 1 (GLUT 1) protein and mRNA
expression in blood-brain barrier (BBB) endothelium. Female rats were
ovariectomized (OVX) for 12-14 days, then E2 was injected sc. at
doses of 1-100 g/kg at 2-16 hours before sampling. Glucose transport
into BBB endothelial cells was assessed using 14C-2-deoxyglucose
(14C-2-DG) uptake. GLUT 1 protein and mRNA samples were analyzed by
Western and Northern blotting, respectively. E2 treatment caused
dose- and time-dependent increases in 14C-2-DG uptake and GLUT 1
protein expression by microvessels. The peak responses were induced
by 10 g/kg E2 dose at the 4-hour sampling time (36.0% and 31.3%
increase, p<0.05, respectively). GLUT 1 mRNA demonstrated a
transient increase at 15-minute (55%, p<0.05), then decreased
to basal level by 2 hours. This study shows that in vivo treatment
with E2 increases 14C-2-DG uptake into the BBB endothelial cells and
suggests this E2 effect is due to its modulation of GLUT 1 mRNA and
protein.
Received 17 July 1996; accepted in final form 23 January 1997.
APS Manuscript Number E342-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 20 February 1997