3-adrenergic-mediated suppression of leptin gene expression in
rats.
Li, Hua, Michael Matheny, and Philip J. Scarpace.
Geriatric Research, Education and Clinical Center, Department of
Veterans Affairs Medical Center, Gainesville, Florida 32608-1197 and
Department of Pharmacology and Therapeutics, University of Florida
College of Medicine, Gainesville, Florida 32610
APStracts 4:0046E, 1997.
To investigate the role of 3-adrenergic receptors in the suppression
of leptin gene expression, we fasted F-344 rats to decrease leptin
mRNA levels, followed by refeeding to stimulate leptin mRNA
production, and examined the ability of the 3-adrenergic agonist,
CGP-12177, to prevent the rise in leptin mRNA levels. In the initial
2 h following CGP 12177 (0.75 mg/kg), there were significant
reductions in both food consumption and in leptin mRNA levels in
epididymal, perirenal, and interscapular WAT. We were unable to
detect leptin mRNA in IBAT, whereas there was a significant increase
in uncoupling protein mRNA levels in IBAT following CGP-12177. The
suppression of leptin mRNA and food intake by CGP-12177 was confirmed
in a second experiment using another rat strain, the F-344xBN.
Furthermore, refeeding after a period of fasting increased leptin
mRNA, which was prevented by CGP-12177. These data indicate a role
for 3-adrenergic-mediated regulation of leptin gene expression in
non-mutant rodents and are consistent with other reports suggesting
that 3-adrenergic agonists suppress food intake.
Received 26 November 1996; accepted in final form 4 February
1997.
APS Manuscript Number E588-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 20 February 1997