Interleukin-8 can mediate acute phase protein production by
isolated human hepatocytes.
Wigmore, Stephen J., Kenneth C. H. Fearon, Jean P. Maingay, M. Phil,
Paul B. S. Lai, Mbchb., James A. Ross.
UNIVERSITY DEPARTMENT OF SURGERY, ROYAL INFIRMARY OF EDINBURGH,
LAURISTON PLACE, EDINBURGH EH3 9YW, UNITED KINGDOM., TEL: ++ 44 - 31
- 536 3827FAX: ++ 44 - 31 - 536 3837
APStracts 4:0131E, 1997.
During the course of studies designed to identify the role of
cytokines in the reprioritization of hepatic protein synthesis
associated with cachexia we detected a hepatocyte-stimulating moiety
in the supernatants of pancreatic cancer cells which was unrelated to
IL-6. This study identifies that moiety as IL-8 and investigates the
role of IL-8 in the induction of acute phase protein production. The
human pancreatic cancer cell line MIA PaCa2 produced >1 ng/ml of
IL-8 per 24 hours and supernatants from this cell line induced C
-reactive protein (CRP) production from isolated human hepatocytes.
Addition of neutralising anti-human IL-8 antibody to such
supernatants produced almost complete inhibition of CRP production.
The addition of recombinant human IL-8 to hepatocytes resulted in a
dose-dependent increase in CRP, [alpha]1-acid glycoprotein and
[alpha]1-antichymotrypsin production and a decrease in the production
of transferrin and pre-albumin. This study demonstrates that
recombinant or tumor-derived IL-8 can modulate acute phase protein
production from isolated human hepatocytes and from human hepatoma
cells.
Received 6 January 1997; accepted in final form 5 June 1997
APS Manuscript Number E002-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 1 July 1997