Ornithine alpha-ketoglutarate modulates tissue protein metabolism
in burn injured rats.
Boucher, Jacques Le, Christiane Obled, Marie-Chantal Farges, and Luc
Cynober .
INSERM U 402, CHU Saint-Antoine, 75571 Paris Cedex 12 ; Laboratoire
d'Etude du M[acute]etabolisme Azot[acute]e, INRA-Theix et CRNH ; and
Laboratoire de Biochimie, Biologie Mol[acute]eculaire et Nutrition,
Facult[acute]e de Pharmacie et CRNH, 63001 Clermont-Ferrand,
France
APStracts 4:0114E, 1997.
Enterally administered OKG displays whole body anabolic and
anticatabolic properties in trauma situations, especially after burn
injury. The aim of this study was to get informations about the
anabolic effect of OKG at tissue level. Thirty six male Wistar rats
(95 +/- 7 g) were allocated to four groups. Eighteen rats were burned
by water (20 % body surface area). After a 24 hours fast (day 0
-day1), rats were enterally refed for 48 hours (day 1-day 3) using
Osmolite_, as a low-calorie low-nitrogen regimen supplemented either
with 5 g OKG/kg/day (B-OKG) or with an equivalent amount of nitrogen
in the form of glycine (B-GLY). Non burned pair-fed controls treated
with glycine (C-GLY) and healthy rats fed ad libitum were also
studied. On day 3, protein synthesis rates (large dose method), free
glutamine concentrations and total protein content were assessed in
tissues. Myofibrillar degradation was assessed by measuring urinary
3-methylhistidine excretion daily from day 0 to day 3. With regard to
tissue protein synthesis rates, we demonstrate for the first time
that OKG displays anabolic properties in the jejunum (FSR in %/day,
ad libitum = 101.9 +/- 4.0; C-GLY = 84.7 +/- 3.1, P < 0.01 vs. ad
libitum; B-GLY = 84.5 +/- 1.6, P < 0.01 vs. ad libitum; B-OKG =
97.5 +/- 3.2, P < 0.05 vs. C-GLY and B-GLY), as well as in the
liver (FSR in %/day, ad libitum = 75.9 +/- 3.7; C-GLY = 53.2 +/- 3.8,
P < 0.01 vs. ad libitum; B-GLY = 70.2 +/- 2.0, P < 0.01 vs. C
-GLY; B-OKG = 98.7 +/- 4.6, P < 0.01 vs. ad libitum, C-GLY and B
-GLY), the latter having previously been observed in vitro.
Furthermore, we confirm that OKG inhibits myofibrillar degradation,
counteracts the trauma-induced fall of muscle glutamine pool and
induces an increase in glutamine concentration in the jejunum.
Received 28 January 1997; accepted in final form 2 May 1997.
APS Manuscript Number E41-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 11 June 1997