Absolute concentrations of glycerol and lactate in human skeletal
muscle, adipose tissue and blood.
Hagstrsm-Toft, Eva, Staffan Enoksson, Erik Moberg, Jan Bolinder, and
Peter Arner .
Department of Medicine and the Research Center and Department of
Vascular Surgery, Huddinge University Hospital, Karolinska Institute,
S-141 86 Huddinge, Sweden
APStracts 4:0115E, 1997.
The absolute concentrations of glycerol and lactate were studied with
microdialysis of adipose tissue and skeletal muscle in normal-weight
subjects. The basal interstitial glycerol concentration was 232+/-33,
96+/-8 and 59+/-6 [mu]mol/l in fat, muscle and arterialized plasma,
respectively (p=0.0002). This relation was maintained during both
euglycemic hyperinsulinemia, when glycerol decreased in all three
compartments and hypoglycemia, when glycerol first decreased, then
increased in fat, muscle and blood (p=0.0001 for both). Basal
interstitial lactate concentrations were similar in adipose tissue
(1.1+/-0.2 mmol/l) and skeletal muscle (1.9+/-0.4 mmol/l) and higher
than in arterialized blood (0.6+/-0.1 mmol/l, p=0.002). During
hyperinsulinemia and hypoglycemia, lactate increased (p=0.0001) and
the tissue/blood relation was maintained (p=0.04). In conclusion,
adipose tissue and skeletal muscle mobilize glycerol and lactate at
rest. Glycerol and lactate production are influenced by
hyperinsulinemia and hypoglycemia in both tissues. Adipose tissue
appears to be the major site of glycerol production, whereas skeletal
muscle and fat may be equally important for lactate production.
Received 24 December 1996; accepted in final form 8 May 1997.
APS Manuscript Number E625-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 11 June 1997