Oxidative metabolism in insulin-treated gestational diabetes
mellitus.
Hsu, Helen W., Nancy F. Butte, William W. Wong, Jon K. Moon, Kenneth
J. Ellis, Peter D. Klein, Kenneth J. Moise.
Department of Obstetrics and Gynecology, USDA/ARS Children's
Nutrition Research Center, Department of Pediatrics, Baylor College
of Medicine and Texas Children's Hospital, Houston, Texas
APStracts 4:0049E, 1997.
To investigate whether protein, carbohydrate and fat metabolism was
normalized in insulin-treated gestational diabetes mellitus (GDM),
eight Hispanic women with GDM and eight healthy controls were studied
at 32-36 weeks of gestation and 6 weeks postpartum. Net substrate
utilization was measured using room respiration calorimetry.
Exogenous substrate oxidation was determined by 13C recovered in
breath CO2 from 13C-labeled leucine, glucose and Hiolein. Women with
GDM had higher 24-hour oxygen consumption, carbon dioxide production,
total energy expenditure and basal metabolic rates than controls due
to larger body mass. Adjusted for weight or fat-free mass, total
energy expenditure and basal metabolic rate, and basal and 24-hour
whole-body net protein, carbohydrate and fat utilization did not
differ between insulin-treated GDM subjects and controls in pregnancy
or postpartum. Oxidation of 13C-leucine and 13C-glucose did not
differ by group or pregnancy status. Recovery of exogenously
administered 13C-Hiolein, a biosynthetic triglyceride, as breath
13CO2 was significantly lower in the GDM group antepartum and
postpartum (P=0.02), indicating lower oxidation of exogenous
triglycerides in GDM.
Received 31 October 1996; accepted in final form 14 February
1997.
APS Manuscript Number E544-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 5 March 1997