Cholate inhibits high-fat diet-induced hyperglycemia and obesity
with acyl-coa synthetase mrna decrease.
Ikemoto, Shinji, Mayumi Takahashi, Nobuyo Tsunoda, Kayo Maruyama,
Hiroshige Itakura, Kentaro Kawanaka, Izumi Tabata, Mitsuru Higuchi,
Tsuyoshi Tange, Tokuo T. Yamamoto, and Osamu Ezaki.
Division of Clinical Nutrition and Health Promotion, National
Institute of Health and Nutrition, Toyama, Tokyo 162, Japan,
Department of Pathology, Faculty of Medicine, University of Tokyo,
Tokyo 113, Japan, Tohoku University Gene Research Center, Sendai 981,
Japan
APStracts 4:0064E, 1997.
The effects of sodium cholate on high-fat diet-induced hyperglycemia
and obesity were investigated. Insulin resistance was estimated by
measuring 2-deoxy glucose uptake in epitrochlearis muscles incubated
in vitro. Addition of 0.5% cholate to high-safflower oil diet
completely prevented high fat-induced hyperglycemia and obesity in
C57BL/6J mice with a slight decrease of energy intake but with no
inhibition of fat absorption. Furthermore, the addition of cholate
decreased blood insulin levels and prevented high-fat diet-induced
decrease of glucose uptake in epitrochlearis. However, there was no
change in the unsaturation index of fatty acids in skeletal muscles
and in GLUT4 levels by cholate. In liver, cholate addition resulted
in cholesterol accumulation and completely prevented high-fat diet
-induced triglyceride accumulation. The changes of triglyceride level
in liver were paralleled to the changes of acyl-CoA synthetase (ACS)
mRNA. ACS catalyses the formation of acyl-CoA from fatty acid, and
acyl-CoA is utilized for triglyceride formation in liver. ACS has a
sterol responsive element 1 (SRE-1) in its promoter region. These
data indicate that the favorable effects of cholate could be partly
the result of down regulation of ACS mRNA.
Received 23 October 1996; accepted in final form 20 February
1997.
APS Manuscript Number E528-6.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 21 March 1997