The perfused rat hindlimb is suitable for skeletal muscle glucose
transport measurements..
Wojtaszewski, Jorgen F. P, Allan B. Jakobsen, Thorkil Ploug, and Erik
A. Richter.
Copenhagen Muscle Research Centre, August Krogh Institute and Dept.
of Medical Physiology, The Panum Institute, Copenhagen University,
Copenhagen, Denmark.
APStracts 4:0205E, 1997.
It has been postulated that the perfused rat hindlimb is unsuitable
for measurements of muscle glucose transport (Hansen et al.,
Am.J.Physiol. 37, C30-C35, 1995). The aim of the present study
therefore was to critically evaluate the suitability of this
preparation for glucose transport measurements using the
extracellular marker mannitol and the glucose analogues 3-O-methyl-D
-glucose or 2-deoxy-D-glucose. In all three muscle fiber types
studied, the rate of 2-deoxy-D-glucose uptake during perfusion was
linear from 1 to 40 minutes during maximal insulin stimulation and
from 1 to 15 minutes during maximal electrical stimulation. Uptake of
2-deoxy-D-glucose was not increased by an increase in perfusate flow.
Combined stimulation with a maximal insulin concentration and
electrical stimulation elicited additive effects on 2-deoxy-D-glucose
uptake in slow and fast twitch oxidative but not in fast twitch
glycolytic muscle fibers. Furthermore, in muscles having high glucose
transport capacities 3-O-methyl-D-glucose is less suitable than 2
-deoxy-D-glucose because of rapidly developing non-linearity of
accumulation. Our findings clearly demonstrate that the perfused
hindlimb is suitable for measurements of muscle glucose transport and
that the most feasible glucose analogue for this purpose is 2-deoxy
-D-glucose.
Received 2 June 1997; accepted in final form 16 September 1997.
APS Manuscript Number E254-7.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 7 October 1997