ABNORMAL ACCESS OF AXIAL VIBROTACTILE INPUT TO DEAFFERENTED SOMATOSENSORY CORTEX IN HUMAN UPPER LIMB AMPUTEES. John JM Kew; Peter W Halligan; John C Marshall; Richard E Passingham; John C Rothwell; Michael C Ridding; C David Marsden and David J Brooks. MRC Cyclotron Unit, Hammersmith Hospital, London, UK, MRC Human Movement and Balance Unit, Institute of Neurology, London, UK Neuropsychology Unit, Radcliffe Infirmary, and Rivermead Rehabilitation, Centre, Oxford, UK, Department of Experimental Psychology, University of Oxford, Oxford, UK.
APStracts 4:0043N, 1997.
ABSTRACT
1. Two human subjects with total deafferentation of one upper limb secondary to traumatic multiple cervical root avulsions were studied. Both subjects developed a supernumerary phantom limb in the early post-injury period, and both subsequently underwent elective, above elbow amputation of the flail, anaesthetic limb. 2. In each subject psychophysical study revealed an area of skin in the anterior pectoral region ipsilateral to the amputation, vibratory or tactile stimulation of which elicited referred sensations (RS) in the phantom limb. This ectopic representation of the phantom limb was similarly responsive to vibrotactile stimulation (VS) and pressure stimulation (PS). 3. The cortical correlates of these referred phantom sensations were explored using positron emission tomographic (PET) measurements of regional cerebral blood flow (rCBF) during VS of the ectopic phantom representation ipsilateral to the amputation. For comparison, rCBF measurements were also made during VS of an homologous part of the pectoral region ipsilateral to the intact arm, stimulation of which was not associated with referred phantom sensations. A voxel-based correlation analysis was subsequently used to study the functional connectivity of activated cortical regions. 4. VS of both pectoral regions was consistently associated with significant activation of the contralateral second somatic sensory (SII) cortex. Additional ipsilateral SII activation was present during VS of both pectoral regions in one of the subjects, and during VS of the pectoral region adjacent to the amputation in the other. In the former subject contralateral thalamic activation was present during VS of both pectoral regions. 5. VS of the pectoral region adjacent to the intact arm was associated with significant activation of the dorsal part of the contralateral primary somatosensory (S1) cortex in a position consistent with the S1 trunk area. In contrast, VS of the ectopic phantom representation in the pectoral region ipsilateral to the amputation was associated with activation of the contralateral S1 cortex which extended from the level of the trunk representation ventrally over distances of 20 mm and 12 mm in the two subjects respectively. 6. The area of S1 cortex significantly activated during VS of the digits in a normal control subject was coextensive with the ventral S1 region abnormally activated during VS of the ectopic phantom representation in the two amputees. This finding suggests that in the amputees the deafferented digit or hand/arm area can be activated by sensory input from the pectoral region. 7. Correlation analysis showed an abnormal pattern of intrinsic connectivity within the deafferented S1 hand/arm area of both amputees. In one subject the deafferented S1 was functionally connected with three times as many S1 voxels as the normally afferented S1. This abnormal functional connectivity extended in both the rostro-caudal and ventro-dorsal dimensions. 8. The results demonstrate that sensory input delivered to the axial body surface may gain access to the S1 hand/arm area in some humans who have suffered extensive deafferentation of this area. They also provide evidence that somatotopic reorganization of S1 may, in part, be related to changes in intracortical connectivity. The findings are consistent with the hypothesis that deafferentation of an area of S1 cortex may result in susceptibility of this area to previously dormant inputs from body surfaces represented in immediate adjacent parts of S1. Alterations in functional connectivity between these adjacent areas of cortex, mediated via deafferentation-induced changes in intracortical GABAergic inhibition, provide a plausible explanation for such plasticity.

Received 6 March 1996; accepted in final form 9 January 1997.
APS Manuscript Number J181-6.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 5 February 1997