Axon conduction and survival in CNS white matter during energy deprivation: A developmental study®MDNM¯. ROBERT FERN, PETER DAVIS, STEPHEN G. WAXMAN, and BRUCE R. RANSOM. Department of Neurology, University of Washington, Seattle, WA98195. Department of Neurology, Yale University School of Medicine, New Haven, CT 06510. Neuroscience Research Center, Department of Veterans Affairs Medical Center, West Haven, CT 16516.
APStracts 4:264N, 1997.
ABSTRACT
We investigated the post-natal development of axon sensitivity to the withdrawal of oxygen, glucose, or the combined withdrawal of oxygen + glucose in the isolated rat optic nerve (a CNS white matter tract). Removal of either oxygen or glucose for 60 min resulted in irreversible injury in optic nerves from adult rats, assessed by loss of the evoked compound action potential (CAP). Optic nerves at ages P50). It is probable that this period of final myelination corresponds to a time of hightened metabolic activity in white matter. The tolerance of CNS white matter to energy deprivation can be categorized into four stages that are correlated with specific developmental features: 1) Premyelination (P0-P4), highly tolerant to anoxia, aglycemia and combined anoxia/aglycemia. 2) Early myelination (P5-P20), partially tolerant of anoxia and aglycemia but not to combined anoxia/aglycemia. 3) Late myelination (P21-P49), very low tolerance of anoxia, aglycemia and combined anoxia/aglycemia. 4) Mature (>P50), low tolerance of anoxia, aglycemia and combined anoxia/aglycemia. The relative resistance of optic nerve function to glucose withdrawal in the presence of oxygen, compared to glucose withdrawal in the absence of oxygen, is presumably due to the presence of oxygen-dependent energy reserves such as astrocytic glycogen, amino-acids and phospholipids.

Received 21 February 1997; accepted in final form 17 September 1997.
APS Manuscript Number J156-7.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1997 The American Physiological Society.
Published in APStracts on 7 October 1997