Mechanical and metabolic determination of v.o2 and fatigue during
repetitive isometric contractions in situ.
Ameredes, Bill T., William F. Brechue, and Wendell N. Stainsby.
Department of Cell Biology and Physiology, University of Pittsburgh
School of Medicine, Pittsburgh, PA 15261; Department of Kinesiology,
Indiana University, Bloomington, IN 47405; Department of Physiology,
University of Florida, Gainesville, FL 32610
APStracts 5:0055A, 1998.
Repetitive isometric tetanic contractions (1/s) of the canine
gastrocnemius-plantaris muscle either were studied at optimal length
(Lo), or short length (Ls0.9.Lo), to determine the effects of initial
length on mechanical and metabolic performance, in situ. Respective
averages of mechanical and metabolic variables were (Lo vs. Ls, all
p=0.05): passive tension (preload) = 55 vs. 6 g/g, maximal active
tetanic tension (Po) = 544 vs. 174 (0.38 x Po) g/g, maximal blood
flow (Q.) = 2.0 vs. 1.4 ml/min x g, and maximal oxygen uptake (V.o2)
= 12 vs. 9 [mu]mol/min x g. Tension at Lo decreased to 0.64 x Po over
20 min. of repetitive contractions, demonstrating fatigue; there were
no significant changes in tension at Ls. In separate muscles
contracting at Lo, Q. was set to that measured at Ls (1.1 ml/min x
g), resulting in decreased V.o2 (7 [mu]mol/min x g), and rapid
fatigue, to 0.44 x Po. These data demonstrate: 1) muscles at Lo have
higher Q. and V.o2 than those at Ls, 2) fatigue occurs at Lo with
high V.o2, adjusting metabolic demand (tension output) to match
supply, and 3) the lack of fatigue at Ls with lower tension, Q., and
V.o2 suggests adequate matching of metabolic demand, set low by short
muscle length, with supply optimized by low preload. These
differences in tension and V.o2 between Lo and Ls groups indicate
that muscles contracting isometrically at initial lengths shorter
than Lo are working under sub-maximal conditions.
Received 18 August 1997; accepted in final form 9 February 1998.
APS Manuscript Number A768-7.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1998 The American Physiological Society.
Published in APStracts on 19 February 1998