Monoclonal antibody p-31 recognizes a novel intermediate filament
-associated protein (p250) in rat podocytes.
Kurihara, Hidetake, Norio Sunagawa, Tatsuo Kobayashi, Kazuhiro Kimura,
Nobuo Takasu, and Tsutomu Shike.
Shionogi Research Laboratories, 5-12-4 Sagisu, Fukushima-ku, Osaka
553, Japan
APStracts 5:0031F, 1998.
The visceral glomerular epithelial cells (GECs) or podocytes of the
renal glomerulus constitute a highly specialized epithelium. To study
the nature of podocytes, we established mouse monoclonal antibodies
against GEC. Clone P-31 reacted exclusively with the cytoplasm of GEC
by immunofluorescence. Immunoblot analysis with P-31 showed that a
single band of 250 kD was detectable in a glomerular lysate. The 250
kD polypeptide (p250) was recovered from TX-100-insoluble fractions
of isolated glomeruli, suggesting that this molecule is associated
with the cytoskeleton. Immunogold staining with P-31 demonstrated
that the gold particles were located at the intersections of
vimentin-type intermediate filaments of podocytes. In developing
kidney, this protein first appeared in immature GECs during the S
-shaped body stage. In puromycin aminonucleoside nephrosis, P-250 was
dramatically increased in glomeruli where enhanced desmin expression
was observed in GECs. These results indicate that P-250 is a novel
intermediate filament-associated protein and plays a role in the
organization of the intermediate filament network in both normal and
diseased conditions.
Received 31 July 1997; accepted in final form 19 January 1998.
APS Manuscript Number F257-7.
Article publication pending Am. J. Physiol. (Renal Physiology).
ISSN 1080-4757 Copyright 1998 The American Physiological Society.
Published in APStracts on 19 February 1998