Ncc27, a homolog of the intracellular chloride channel p64, is
expressed in the brush border of renal proximal tubule.
Tulk, Barry M., and John C. Edwards.
Department of Medicine, Washington University School of Medicine,
St. Louis MO 63110
APStracts 5:0042F, 1998.
NCC27, a 27 kDa homolog of the intracellular chloride channel, p64,
was recently described as a chloride channel in nuclear membrane. We
probed human northern blots for NCC27 and found an approximately 1.7
kB message in all tissues examined including kidney, the transcript
being most abundant in heart and skeletal muscle. NCC27-specific
antisera was raised to a C-terminal peptide derived from the NCC27
coding region. Using this antisera, we find NCC27 is expressed in an
intracellular vesicular compartment in HeLa cells, PancI cells, and
macrophages. In human and mouse kidney, NCC27 is expressed at low
levels in most cells of the kidney. NCC27 is highly expressed in
glomeruli, in peri-arterial smooth muscle, and in the apical membrane
of a subset of cortical tubule cells. Double staining with nephron
segment specific lectins indicate that the NCC27-expressing cells are
proximal tubule.
Received 24 September 1997; accepted in final form 12 February
1998.
APS Manuscript Number F310-7.
Article publication pending Am. J. Physiol. (Renal Physiology).
ISSN 1080-4757 Copyright 1998 The American Physiological Society.
Published in APStracts on 19 February 1998