Anomalous decrease in dextran sulfate clearance in the diabetic rat kidney. Burne, Melissa J., Yal_in Adal, Neale Cohen, Sianna Panagiotopoulos, George Jerums, and Wayne D. Comper. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia, 3168. Endocrine Unit, Austin and Repatriation Medical Centre, Department of Medicine, University of Melbourne, Heidelberg, Victoria, Australia, 3084
APStracts 5:0007F, 1998.
The anomalous increase in charge selectivity as previously observed with reduced dextran sulfate clearances in diabetic rats (Michels et al (1982) Kidney Int 21:699-705) was confirmed in 4 week streptozotocin (STZ) diabetic Sprague-Dawley rats using the isolated perfused kidney technique. The apparent charge selectivity in both control and diabetic rats could be abolished by increasing the dextran sulfate concentration to 200 (g/ml in the perfusate. This demonstrated a high rate of processing of dextran sulfate (1,700 ng/min.kidney) by glomeruli in both control and diabetic kidneys and the fact that charge interaction could not explain the concentration dependence. The amount of urinary desulfation of dextran sulfate was also found to be significantly less in the diabetic kidney as was glomerular sulfatase activity compared to controls. Dextran sulfate glomerular processing is therefore altered in the STZ-diabetic rat kidney but could be rationalised in terms of previous models of endothelial cell receptor-mediated uptake of dextran sulfate. The results are consistent with recent work demonstrating that there is little or no electrostatic charge interaction operating on dextran sulfate or other negatively charged molecules at the glomerular capillary wall. 

Received 3 September 1997; accepted in final form 5 January 1998.
APS Manuscript Number F284-7.
Article publication pending Am. J. Physiol. (Renal Physiology).
ISSN 1080-4757 Copyright 1998 The American Physiological Society.
Published in APStracts on 28 January 1998