Morphological and functional characterization of a conditionally
immortalized collecting tubule cell line.
Takacs-Jarrett, Marcia, William E. Sweeney, Ellis D. Avner, and Calvin
U. Cotton.
Departments of Pediatrics, and Physiology and Biophysics, Case
Western Reserve University, Cleveland, OH 44106-4948
APStracts 5:0139F, 1998.
A conditionally immortalized collecting tubule cell line, mCT1, was
derived from the H-2Kb-ts A58 transgenic mouse (ImmortoMouse) which
harbors a temperature- sensitive mutant of the SV40 large T antigen
oncogene. Cells maintained under permissive conditions (33oC with
interferon-, IFN- ) form epithelial monolayers, express large T
antigen, and proliferate (>50 passages). The cells retain
properties characteristic of the renal collecting tubule including:
vasopressin-stimulated cAMP accumulation, aquaporin 2 expression,
high transepithelial electrical resistance, vasopressin-stimulated
ion transport, and amiloride-sensitive sodium absorption. When the
cells are transferred to nonpermissive conditions (39oC without IFN-
) the steady- state level of large T antigen protein declines (>95%
decrease) and cell proliferation is arrested. This conditionally
-immortalized, murine renal cell line should prove useful for studies
of collecting tubule physiology and large T antigen biology.
Received 27 August 1997; accepted in final form 5 August 1998.
APS Manuscript Number F278-7.
Article publication pending Am. J. Physiol. (Renal Physiology).
ISSN 1080-4757 Copyright 1998 The American Physiological Society.
Published in APStracts on 21 September 1998